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pcon planner pro 6.7 crack.Effects of a novel monoclonal antibody, E5, directed against human epidermal growth factor receptor-2 on the biology of SKBR3 cells.
We investigated the effects of E5, a novel humanized monoclonal antibody directed against the extracellular domain of the human epidermal growth factor receptor-2, on the proliferation, cell cycle progression and apoptosis of SKBR3 breast cancer cells. SKBR3 cells were treated with E5 or control IgG for 16 h, and the effects on cell proliferation, cell cycle progression and apoptosis were determined. E5 inhibited SKBR3 cell proliferation in a concentration-dependent manner. The number of cells in the G(0)/G(1) and S phase was reduced by more than 50% after the cells were exposed to 5.0 microg/ml of E5 for 16 h. Furthermore, the cells treated with 5.0 microg/ml of E5 were arrested at the G(0)/G(1) phase. In contrast, 5.0 microg/ml of E5 only slightly affected the proliferation of SKBR3 cells, and the number of cells in the S phase was only slightly reduced. Flow cytometry showed that E5 induced apoptosis in the SKBR3 cells. Furthermore, caspase-3 activity was markedly increased in the E5-treated cells compared to control cells. These results indicate that E5 inhibited the growth of SKBR3 cells by blocking cell proliferation at the G(0)/G(1) phase. E5-mediated apoptosis of SKBR3 cells is strongly associated with the activation of caspase-3.Follow-up of the percutaneous treatment of Crohn's disease.
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