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May 20, 2010 In response to a detailed questionnaire, 31% of medical school graduates and medical students. Publications by the Division of General Internal Medicine - Westchester Medical Center (1977–2007)
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To investigate the distribution of integrin beta 1 and the cytoskeleton organization in human umbilical vein endothelial cells (HUVEC) during the dynamic process of tube formation. HUVEC were cultured on glass coverslips pre-coated with collagen type I. The actin filament was stained by rhodamine-phalloidin and the cell surface receptor alpha 5 beta 1 of HUVEC was detected by immunofluorescence. Integrin beta 1 in HUVEC was also detected by immunogold electron microscopy. After incubating HUVEC on collagen type I for 2 hours, the cells extended and formed filopodia. The initial aggregations of actin filaments (cell membrane) and integrin beta 1 appeared in the area of cell-cell contact after 15 min incubation. The filopodia became thicker, and the contractile filaments of actin was found in the leading edge of the cells and the cells aggregated to form a net. The filopodia and cytoskeleton-mediated cell-cell contact were identified after 2 hours incubation. The amount of filopodia peaked and was localized at the tip of the cell. The tube formation of HUVEC was terminated after 18 hours incubation. Immunogold electron microscopy showed 82fe58eafd
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