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The present study was designed to investigate the antidiabetic activity of polysaccharide isolated from the seeds of Amaranthus hypochondriacus (AHPS), a commonly used Chinese herbal medicine. AHPS was prepared by hydrolysis and deproteinization of A. hypochondriacus seeds. An acute- and subacute-trials were conducted to evaluate antidiabetic effects of AHPS on the streptozotocin (STZ)-induced diabetic rat. The diabetic rats were induced by single intravenous injection of 65 mg/kg STZ in citrate buffer. After 2 months, the blood glucose levels, lipid levels, and serum insulin level were determined. The results showed that AHPS significantly decreased the blood glucose levels and increased the serum insulin level. AHPS also ameliorated lipid profiles. These results indicated that AHPS significantly protected rats from STZ-induced diabetes. For the examination of AHPS antidiabetic mechanisms, an in vitro study was conducted to determine glucose uptake ability of AHPS-treated 3T3-L1 adipocytes by the [³H] 2-deoxy-glucose uptake assay. AHPS significantly stimulated glucose uptake in a dose-dependent manner in 3T3-L1 adipocytes. In addition, the efficacy of AHPS on insulin receptor phosphorylation and intracellular signaling was investigated in the absence and presence of insulin using immunoblotting analysis. The results demonstrated that AHPS enhanced tyrosine phosphorylation of insulin receptor and subsequent downstream signaling, which were triggered by insulin. The results of the present study suggested that AHPS possessed antidiabetic activity, which was mediated by stimulation of glucose uptake, augmentation of insulin action, and activation of insulin signaling pathways.How to Find a Great Lawyer
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