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Cl- transport systems in renal medullary thick ascending limb (mTAL) cells provide an entry pathway for blood volume expansion. Here, we performed biochemical and electrophysiological studies to determine the properties of Cl- transport systems in mTAL cells. mTAL cells were enzymatically isolated from Sprague-Dawley rats. Each cell was suspended in salt solution, and Cl- transport was estimated by measuring short-circuit current (I(sc)) using a modified Ussing chamber. Additionally, the transport activity was examined by measuring the uptake of intracellular Cl-. The vesicles from mTAL cells showed inwardly directed Cl- flux, which was completely inhibited by the Cl- channel blocker, anthracene-9-carboxylic acid. The transport activity of mTAL cells was highly dependent on extracellular Cl-. The influx of Cl- was saturated by extracellular Cl-, but not by the physiological cation, Na+. The transport of Cl- in mTAL cells was electrically coupled and was inhibited by 0.1 microM 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid and 100 microM 5-nitro-2-(3-phenylpropylamino)benzoate. These results indicated the presence of a Cl- transport system on the cell membrane. In contrast to the physiological cation, Na+, and the organic cation, 1-N-methyl-4-phenylpyridinium, mTAL cells failed to increase intracellular Ca2+ concentration in response to hypotonic conditions. These results indicate that incomplete osmotic regulation is important for maintaining cell volume in mTAL cells.The present invention generally relates to a grinding machine, and more particularly, to a method and system for providing a grinding wheel having a desired profile.
In the field of surface finishing, a finished surface having a desired profile is sometimes desired. Machining of a surface may result in a surface having a profile that is not the desired profile. For example, a surface
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