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After the installation process you need to complete registration procedure in order to use this program fully.Scrapie pathogenesis: kinetics of prion protein accumulation and PrPSc accumulation in the ovine medulla oblongata.
The central nervous system (CNS) is one of the main targets of prion infection. In humans, prion infection of the CNS is associated with spongiform encephalopathy (TSE), which is a fatal neurodegenerative disorder. Although several animal species have been reported to suffer from TSE, disease transmission in sheep has been difficult to demonstrate. In this work, we analyzed the kinetics of PrP accumulation during scrapie in the ovine medulla oblongata, a CNS region largely unexplored in scrapie research. The study was performed in two naturally scrapie-infected flocks. In the first experiment, scrapie-infected animals were euthanized on the first, second, or fourth day of disease. The investigation involved cryoprotection, PrP immunohistochemistry, and Western blot of brain tissues. In the second experiment, scrapie-infected animals were euthanized every week, and the analysis was repeated. Regarding the first experiment, PrP accumulation did not change significantly throughout the preclinical period of the disease. This observation was consistent for all animals. However, we found that the accumulation of PrPSc in the medulla oblongata was related to the progress of the disease. The prion accumulation increased after the appearance of clinical signs. In the second experiment, PrP accumulation also increased after the appearance of clinical signs. Data indicate that the ovine medulla oblongata could be considered an optimal site for studying the prion protein pathogenesis. The results of this work contribute to elucidate the molecular pathogenesis of the disease.Q:
What is the difference between image 1st, 2nd, 3rd pincode barcode?
What is the difference between 1st, 2nd, 3rd pincode barcode?
A:
Basically the first is a barcode that's 1D: it is read horizontally from left to right across the barcode (along the black/white line).
The second is a 2D barcode that is read vertically from bottom to top across the barcode.
The third is even more complicated, as it's a multidimensional code. 
Barcode encoding explains them on Wikipedia.
Scott
26-May-2015 07:55
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