El Libro BLANCO RAMTHA.pdf
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Papaya ringspot virus (PRSV) is a bipartite begomovirus transmitted by the whitefly Bemisia tabaci and has a circular, single-stranded DNA genome of approximately 2.1 kbp. The gene for coat protein (CP) is encoded by the single-stranded DNA genome and is expressed in the cytoplasm of infected cells. The recombinant N-terminal and C-terminal domains of the coat protein were expressed in E. coli and used to immunize mice. A panel of 20 monoclonal antibodies (MAbs) was generated using hybridoma technology and used to identify epitopes on the coat protein. MAbs could be divided into three different groups based on the reactivity patterns in ELISA and Western blot analyses. Three distinct epitopes were identified using N-terminal and C-terminal domains of coat protein.Airway alterations induced
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