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We developed a convenient procedure for isolating peptide amines and amides from peptide mixtures using (2)H, (15)N-labeled peptide moieties. For this, (2)H-labeled peptides were first isolated by HPLC in the presence of a fluorescent tag, and (2)H-labeled amino acids were subsequently introduced into the isolated peptide by hydrolysis of the fluorescent tag. The fluorescence of the purified peptide was then used for its identification. This method allowed us to characterize peptide amines and amides from peptide mixtures in a single HPLC run. The suitability of this method was demonstrated using tryptophan-containing peptide mixtures, and we successfully identified various peptide amines and amides including tryptophan. In addition to the above characterization, we also found that this method is also applicable for the isolation of histamine and histidine amine from peptide mixtures.Detroit and the SEC
Brandon McManus has some pretty loaded words for the SEC regarding the future of the Big Ten. He views them as a possible threat, as they attempt to expand into the Midwest and West. He says SEC TV networks will struggle trying to compete with the Big Ten's $9.7 billion media rights deal. On the subject of the Big Ten's Detroit-based media rights, I have to agree with McManus, although Michigan fans will still have a chance to compete.
"They have plenty of money and they can build their own channel... but they really need to find a way to get current football games to their fans," McManus said in a column for MSN.com.
The Big Ten has been taking a good deal of heat for its lack of support of the Wolverines in that state. They've been using their new College Football Playoff media deal as an excuse to say there aren't enough TV markets in the Midwest and Western U.S.
But,
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Uploading a document to Firestore database in Flutter
I'm trying to upload a PDF to my Firestore database. This code segment is where I'm trying to do it
StorageReference profilePicRef = FirebaseStorage.instance
   .referenceFromURL(Uri.parse('File://' + path.join('assets', 'profilePicture.png')));
Image image = Image.file(profilePicRef, file.readAsBytesSync());
profilePicture = userCollection.document().setData({"profilePicture": image});
And then on the client side I have this:
DatabaseReference storageRef = FirebaseDatabase.instance.reference().child("users");
Map userMap = new Map();
userMap["profilePicture"] = profilePicture;
DatabaseReference documentReference = storageRef.push();
documentReference.setData(userMap);
print('user added to Firestore');
When I run the app this is what happens
[MY-APP] Uploading file (2MB)
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