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The antibiotic gentamicin is generally used to treat infections caused by Pseudomonas aeruginosa, which is inherently resistant to this drug. To determine the basis of gentamicin resistance in P. aeruginosa, a reverse genetic method for isolating gentamicin-induced genes was developed. A gentamicin-based selection method was developed to select for P. aeruginosa strains exhibiting increased efflux activity, as determined by an increase in their ability to export rhodamine 6G. The two gentamicin-selected mutants isolated were independent of a known aminoglycoside efflux transporter. To detect strains containing transposons, which could mimic drug-induced mutations, the two gentamicin-selected strains were tested for susceptibility to other aminoglycosides, rifampin, and ciprofloxacin. Their gentamicin MICs were

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