Powerofcriticalthinkingvaughn4theditionpdf
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[Functional analysis of the effects of Mg2+ on dengue virus RNA-dependent RNA polymerase activity].
To study the functional mechanism of dengue virus RNA-dependent RNA polymerase (DENV RdRP) inhibition by magnesium, the biochemical properties of DENV-2 RdRP with respect to magnesium and the effects of Mg(2+) on DENV-2 RdRP enzymatic activity and the conformation of the enzyme were investigated. The enzymatic activity was assayed by using recombinant plasmid containing DENV-2 complementary RNA and [α-32P]-UTP as substrate. The kinetic parameters of the reaction were calculated. The conformational changes of the enzyme in the presence of Mg(2+) were detected by intrinsic fluorescence analysis. DENV-2 RdRP showed highest activity at pH 7.5 and 5 °C. At 30 °C, the enzyme activity was about 10-fold lower than that at pH 7.5. The reaction rate was dependent on the Mg(2+) concentration. The enzymatic activity of DENV-2 RdRP was promoted by Mg(2+), with a K(m) of 3.5 μmol/L and a V(max) of 2.07 × 10(-4) μmol/L/min. With a concentration ranging from 0.5 to 6.0 mmol/L, Mg(2+) increased the fluorescence intensity in the fluorescence analysis. Mg(2+) probably binds to the enzyme and changes the conformation of the enzyme. It increases the RdRP enzymatic activity and improves the catalytic efficiency of the enzyme. Mg(2+) can enhance the activity of DENV-2 RdRP, which may play a role in the replication process of DENV-2.Q:
How to determine the number of records in a CLOB
I need to store data in a CLOB field in a table. Is there a way to determine how many records there will be in the CLOB field, other than looping through
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