ThepathansbyolafcaroePDF
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Evidence for subunit interaction in glucose transport by human red blood cells.
The interactions of the three major glucose transport systems (glucose carriers) in human red blood cells have been investigated by analyzing the effects of changes in extracellular pH and intracellular Mg2+ and glucose concentration on the rates of glucose uptake and the levels of carrier protein present in a 4-hr incubation at 37 degrees C. Exchangeable or non-exchangeable sugars competed with glucose for uptake in all the experiments. A major change in the Km of the transport system for glucose was observed in the presence of intracellular Mg2+ at low pH. Maximal transport rates in the presence of both Mg2+ and glucose (0.5 mM) were about 60% lower than those seen in the absence of Mg2+, which suggests the presence of a low-affinity site. An increase in intracellular Mg2+ caused a large increase in Km at pH 7.4, but only a small change in Km at pH 6.2. Similar effects of pH were observed on carrier protein levels. There was no change in Km at low intracellular pH in the presence of Mg2+. The data are consistent with the view that all three carriers are present in a complex with several different subunits, and that one or more of these subunits may be Mg2+-binding.Applying a "Fractal Coefficient" to estimate bacterial survival after deep hypothermic storage.
Survival of bacteria in deep hypothermic storage is a phenomenon that is governed by a combination of cellular response to a given insult and the propensity of a given microorganism to survive a given insult. The focus of this study is to provide a framework for the determination of "best
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