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'cause she's a thief
She steals all my love
I don't know why
I feel the need
I gotta get away
She steals all my life
I'm the girl, he's the guy
He makes me feel like I've been in love before
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And she takes my man
I can't believe it
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She takes all I want to give
I gotta get awayMigration of bone marrow-derived dendritic cells in the human gastrointestinal tract.
Dendritic cells (DC) have an essential role in mucosal immunity and are present in all mucosal tissues. However, their role in the human gastrointestinal (GI) tract is poorly defined. We investigated the migration of bone marrow-derived DC (BMDC) in the human GI tract using in vitro and in vivo models. BMDC migration was analyzed using a skin window chamber and a Ussing chamber model, in which DC migrate along the epithelium. The distribution of BMDCs was analyzed by immunohistochemistry. T cells were activated in vitro with mucosal antigen and cocultured with BMDCs. T cells that were primed with BMDCs were transferred into T cell-deficient nude mice, and the proliferation of transferred T cells was analyzed. Inducible nitric oxide synthase (iNOS) expression was assessed by immunohistochemistry. Migration of DC was analyzed by immunohistochemistry in each mucosal layer and was associated with antigen-specific T cells. Furthermore, iNOS expression in T cells was detected by confocal laser scanning microscopy. Finally, migration of BMDC was also evaluated by transferring BMDC-labeled DC and performing dual immunohistochemistry. BMDCs migrated in the epidermis, submucosal, and mucosal layers. T cells were induced by mucosal antigen via direct contact with DC, and BMDCs were recruited to the draining lymph nodes. However, they did not migrate to the mesenteric lymph nodes. Immunohistochemistry revealed that iNOS was expressed in CD4- and CD8-positive T cells that interacted with BMDCs. BMDCs that expressed i 3125841983

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