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2006[50]. (2007)[10] was the year when,Hollow Man (2000) sci-fi,thriller movie is released.
2016[11]. (2017)[12] was the year when,Hollow Man (2000) sci-fi,thriller movie is released.
We hope, you enjoyed our work so please and share it on your social media accounts.MicroRNA profiles in different stages of hepatocellular carcinoma and their potential targets.
MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level. Aberrant miRNA expression profiles are associated with different types of cancers, including liver cancer. To identify the most differentially expressed miRNAs in HCC, miRNA expression profiles in a training set of 9 cases of liver cancer and 10 normal livers were obtained by a microarray analysis. A further validation set of 21 cases of HCC and 10 normal livers was used for confirmation of differentially expressed miRNAs. Ten potential target genes of miR-338-3p and miR-490-3p were identified by bioinformatics analysis and verified by dual-luciferase reporter assay. The expression of miR-338-3p and miR-490-3p was significantly downregulated in HCC, and were remarkably associated with the TNM stage and histological grade of HCC. Further validation of HCC and normal liver tissues with RT-PCR confirmed the accuracy of the microarray data. Bioinformatics analysis indicated that gene CDK4, a key regulator of cell proliferation, was a target of miR-338-3p and miR-490-3p. Gene CDK4 was downregulated in HCC. Dual-luciferase reporter assay indicated that gene CDK4 was negatively regulated by miR-338-3p and miR-490-3p in HCC cells. The downregulation of miR-338-3p and miR-490-3p was significantly associated with advanced HCC stages and poor HCC histological grades. MiR-338-3p and miR-490-3p regulate the target gene CDK4 in HCC, and are involved in the progression of HCC.Q:
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