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Formation of desmethyldiazepam in plasma by hepatic microsomal metabolism of diazepam.
The role of the liver in the metabolism of diazepam was investigated in male Swiss mice. After intravenous administration of 14C-diazepam in a dose of 0.5 mg/kg, over 90% of radioactivity was recovered in plasma within 30 min. The radioactivity in plasma was equally distributed between diazepam and desmethyldiazepam. The blood elimination half-life of diazepam was found to be about 2.4 h, whereas that of desmethyldiazepam was only 0.5 h. The metabolism of diazepam in liver was studied in male mice pre-treated with phenobarbital, acetone or ethanol. After diazepam was given intraperitoneally, liver microsomes were prepared from control, acetone-, and ethanol-pretreated animals and incubated with diazepam in the presence of NADPH and glutathione. The ratio of desmethyldiazepam to diazepam formed was approximately 2.5 in control animals, but reached up to 25 in mice pretreated with acetone and ethanol. When diazepam was incubated with rat liver microsomes in the presence of NADPH, desmethyldiazepam was also formed. These results indicate that the liver plays an important role in the formation of desmethyldiazepam from diazepam.[Growth factors and wound healing].
Growth factors are polypeptides acting at the level of the gene expression, protein synthesis, or signal transduction. Growth factors are essential in normal tissue development and wound repair. The action of growth factors appears to be coordinated at two distinct levels. The first level is the control of proliferation and differentiation by the interaction between specific growth factors and their receptors. The second level is the control of their synthesis by regulation of gene expression. This review focuses on the actions of several growth factors during normal tissue repair, particularly those involved in wound healing.The National Basketball Association (NBA) is moving towards putting the broadcast rights for its 2019-20 season to a streaming service, people familiar with the matter told Bloomberg.
The streaming service will be owned by a company owned by the group behind Netflix, which will be named after the NBA itself, according to the people, who asked not to be identified because the negotiations are private. The move could be announced as early as next week, though
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